WEKO3
アイテム
Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy
https://ocu-omu.repo.nii.ac.jp/records/2021233
https://ocu-omu.repo.nii.ac.jp/records/20212332406debb-05e4-45e9-9958-58a07cf64e1a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (55.9 KB)
|
|
| Item type | 共通(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-01-15 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy | |||||||||||
| 言語 | en | |||||||||||
| 作成者 |
Tulum, Isil
× Tulum, Isil× 田原, 悠平× 宮田, 真人
KAKEN2
1000050209912
|
|||||||||||
| 権利情報 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | This article has been accepted for publication in Microscopy Published by Oxford University Press. The version of record is available free of charge on Microscopy website at: https://doi.org/10.1093/jmicro/dfz033. | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | リゾチーム | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 蛍光 | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | ペニシリン | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | パイクラスト | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | L型 | |||||||||||
| 主題 | ||||||||||||
| 言語 | ja | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 膨圧 | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | lysozyme | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | fluorescence | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | penicillin | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | piecrust | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | L-form | |||||||||||
| 主題 | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | turgor | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Peptidoglycan, which is the main component of the bacterial cell wall, is a heterogeneous polymer of glycan strands cross-linked with short peptides and is synthesized in cooperation with the cell division cycle. Although it plays a critical role in bacterial survival, its architecture is not well understood. Herein, we visualized the architecture of the peptidoglycan surface in Bacillus subtilis at the nanometer resolution, using quick-freeze, deep-etch electron microscopy (EM). Filamentous structures were observed on the entire surface of the cell, where filaments about 11 nm wide formed concentric circles on cell poles, filaments about 13 nm wide formed a circumferential mesh-like structure on the cylindrical part and a 'piecrust' structure was observed at the boundary. When growing cells were treated with lysozyme, the entire cell mass migrated to one side and came out from the cell envelope. Fluorescence labeling showed that lysozyme preferentially bound to a cell pole and cell division site, where the peptidoglycan synthesis was not complete. Ruffling of surface structures was observed during EM. When cells were treated with penicillin, the cell mass came out from a cleft around the cell division site. Outward curvature of the protoplast at the cleft seen using EM suggested that turgor pressure was applied as the peptidoglycan was not damaged at other positions. When muropeptides were depleted, surface filaments were lost while the rod shape of the cell was maintained. These changes can be explained on the basis of the working points of the chemical structure of peptidoglycan. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Grant-in-Aid for Scientific Research on the Innovative Area 'Harmonized Supramolecular Motility Machinery and Its Diversity' (MEXT KAKENHI grant number 24117002); Grant-in-Aids for Scientific Research (B) and (A) (MEXT KAKENHI grant numbers 24390107 and 17H01544); Osaka City University (Strategic Research Grant 2019 to M.M.); a Grant-in-aid of the FUGAKU TRUST FOR MEDICINAL RESEARCH to M.M. / 日本顕微鏡学会第36回(2021年度)論文賞 | |||||||||||
| 言語 | ja | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Oxford University Press | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 記事種別等 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Article | |||||||||||
| 言語 | en | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_8042 | |||||||||||
| 資源タイプ | working paper | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | NA | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||
| 識別子 | ||||||||||||
| 識別子 | https://doi.org/10.1093/jmicro/dfz033 | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連情報 | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1093/jmicro/dfz033 | |||||||||||
| 書誌情報 |
en : Microscopy 巻 68, 号 6, p. 441-449, 発行日 2019-11-05 |
|||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 2050-5701 | |||||||||||
