{"created":"2024-10-31T02:39:15.140634+00:00","id":2020482,"links":{},"metadata":{"_buckets":{"deposit":"2d1718d4-854a-41dc-9675-42e2b47cfe8f"},"_deposit":{"created_by":7,"id":"2020482","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020482"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020482","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Alcohol abrogates human norovirus infectivity in a pH-dependent manner","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University, Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Sato, Shintaro","creatorNameLang":"en"},{"creatorName":"サトウ, シンタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"佐藤, 慎太郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000080447333","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000080447333"},{"nameIdentifier":"0000-0001-5156-3354","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-5156-3354"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Matsumoto, Naomi","creatorNameLang":"en"},{"creatorName":"マツモト, ナオミ","creatorNameLang":"ja-Kana"},{"creatorName":"松本, 直実","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Hisaie, Kota","creatorNameLang":"en"},{"creatorName":"ヒサイエ, コウタ","creatorNameLang":"ja-Kana"},{"creatorName":"久家, 広大","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Uematsu, Satoshi","creatorNameLang":"en"},{"creatorName":"ウエマツ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"植松, 智","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050379088","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050379088"},{"nameIdentifier":"ue1","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/ue1"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2020 Author(s). ","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"ヒトノロウイルス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"手指消毒薬","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"アルコール","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"抗ウイルス活性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Public health","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Virology","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究グループは、自ら確立したヒトiPS細胞株由来腸管上皮細胞を用いたヒトノロウイルス増殖系を用いて、pHを酸性、もしくはアルカリ性に傾けた消毒用アルコールに、ヒトノロウイルスをほぼ完全に不活化しうる効果があることを確認しました。pHの調整には食品添加物として用いられるクエン酸(市販のレモン果汁でも可)や重曹を用いることができるため、今回の研究成果は、抗ヒトノロウイルス活性を持つ手指消毒薬の開発、検証に役立つことが期待されます。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Alcohol-based disinfectants are widely used for the sanitization of microorganisms, especially those that cause infectious diseases, including viruses. However, since the germicidal mechanism of alcohol is lipolysis, alcohol-based disinfectants appear to have a minimal effect on non-enveloped viruses, such as noroviruses. Because there is no cultivation method for human norovirus (HuNoV) in vitro, murine norovirus and feline calicivirus have been used as surrogates for HuNoV to analyze the efficacy of disinfectant regents. Therefore, whether these disinfectants and their conditions are effective against HuNoVs remain unknown. In this study, we report that ethanol or isopropanol alone can sufficiently suppress GII.4 genotype HuNoV replication in human iPSC-derived intestinal epithelial cells. Additionally, pH adjustments and salting-out may contribute toward the virucidal effect of alcohol against other HuNoV genotypes and cancel the impediment of organic substance contamination, respectively. Therefore, similar to sodium hypochlorite, alcohol-based disinfectants containing electrolytes can be used for HuNoV inactivation.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"The project was supported by Grants from the Japan Society for the Promotion of Science (Grant-in Aid for Scientific Research (C) [16K08836 to S.S.], Scientific Research (B) [19H03702 to S.S.], and Challenging Research (Exploratory) [19K22532 to S.S.]); Japan Agency for Medical Research and Development (AMED) [20fk0108122s0302 to S.S.]; and the Mochida Memorial Foundation for Medical and Pharmaceutical Research (to S.S).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1038/s41598-020-72609-z"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-28","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"15878","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-09"}],"displaytype":"detail","filename":"20452322-10-15878.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020482/files/20452322-10-15878.pdf"},"version_id":"e8d70c87-5eda-48b0-a682-08d730e3f14a"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Alcohol abrogates human norovirus infectivity in a pH-dependent manner","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-10-09"},"publish_date":"2020-10-09","publish_status":"0","recid":"2020482","relation_version_is_last":true,"title":["Alcohol abrogates human norovirus infectivity in a pH-dependent manner"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T02:39:24.712552+00:00"}