{"created":"2024-10-31T02:36:37.968206+00:00","id":2020452,"links":{},"metadata":{"_buckets":{"deposit":"bafe22cf-2bdc-4f5b-b2ec-09e01494c9f2"},"_deposit":{"created_by":7,"id":"2020452","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020452"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020452","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Fujita, Ken-Ichi","creatorNameLang":"en"},{"creatorName":"フジタ, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"藤田, 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俊雄","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010137185","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010137185"},{"nameIdentifier":"read0020847","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/read0020847"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is the accepted manuscript version of an article published in Polymer Journal. The final authenticated version is available online at: https://doi.org/10.1038/s41428-020-00413-7. See Springer Nature terms for use of archived author accepted manuscripts of subscription articles. https://www.nature.com/nature-research/editorial-policies/self-archiving-and-license-to-publish","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"納豆","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリグルタミン酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"枯草菌","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus sp.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Biopolymer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Poly(γ-glutamic acid)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"size exclusion chromatography in conjunction with multiangle laser light scattering","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"納豆のネバネバの主因であるポリグルタミン酸(PGA)は、枯草菌の一種である納豆菌が分泌生産する高分子です。これまで、PGA生合成に必須なタンパク質は、PGA合成関連pgsオペロンから翻訳されるPgsB、PgsC、およびPgsAと報告されてきました。本研究では、pgsオペロンにおいてpgsAの下流に位置するpgsE遺伝子に着目し、PgsEタンパク質がPGAの生合成に与える影響を動的粘度法や多角度光散乱検出器による分子量解析を通して調べました。その結果、PgsB、PgsC、およびPgsAの3つのタンパク質で生産されたPGAは、ほとんど粘性を示さず、47,000の分子量でした。一方、PgsB、PgsC、PgsA、およびPgsEの4つのタンパク質で生産されたPGAは高粘性を示し、その分子量も2,900,000であることが判明しました。以上の結果より、PgsEは、PGAの分子量を大幅に向上させ、納豆をネバネバにさせる主因である可能性が示唆されました。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Poly(γ-glutamic acid) (PGA) is a biopolymer produced by Bacillus spp. via the γ-amide linkages of D- and/or L-glutamate. Although high-molecular-weight (HMW) PGA possesses many attractive properties, such as flocculating, wound healing, and immune-stimulating effects, no studies have reported factors useful for increasing the molecular weight of PGA during microbial production. PgsB, PgsC, and PgsA are the minimum protein sets required for PGA production in B. subtilis, and PgsE improves PGA productivity. Analysis by size-exclusion chromatography combined with multiangle laser light scattering revealed that the molecular weight of PGA was M_w = 2,900,000 g mol^−1 and predominantly M_w = 47,000 g mol^−1 in preparations derived from B. subtilis cells with and without pgsE, respectively. PgsE may be required to increase the molecular weight of PGA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1038/s41428-020-00413-7"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1349-0540","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"414","bibliographicPageStart":"409","bibliographicVolumeNumber":"53","bibliographic_titles":[{"bibliographic_title":"Polymer Journal","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41428-020-00413-7","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"AA12453460","subitem_relation_type_select":"NCID"}}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-04-06"}],"displaytype":"detail","filename":"13490540-53-2-409.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020452/files/13490540-53-2-409.pdf"},"version_id":"ec559f23-bc79-4687-b2d8-9f0d02ae40ec"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Note","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-04-06"},"publish_date":"2021-04-06","publish_status":"0","recid":"2020452","relation_version_is_last":true,"title":["Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T02:36:47.047096+00:00"}