{"created":"2024-10-31T02:36:03.434315+00:00","id":2020446,"links":{},"metadata":{"_buckets":{"deposit":"66e03f66-d942-45bf-a762-5fa30703e61f"},"_deposit":{"created_by":7,"id":"2020446","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020446"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020446","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Shimatani, Akiyoshi","creatorNameLang":"en"},{"creatorName":"シマタニ, アキヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"嶋谷, 彰芳","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0002-5333-5184","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5333-5184"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Toyoda, Hiromitsu","creatorNameLang":"en"},{"creatorName":"トヨダ, ヒロミツ","creatorNameLang":"ja-Kana"},{"creatorName":"豊田, 宏光","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050514238","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050514238"},{"nameIdentifier":"0000-0002-3126-0046","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-3126-0046"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Orita, Kumi","creatorNameLang":"en"},{"creatorName":"オリタ, クミ","creatorNameLang":"ja-Kana"},{"creatorName":"折田, 久美","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000040748597","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000040748597"},{"nameIdentifier":"0000-0003-3291-2509","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-3291-2509"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Ishikiriseiki Hospital","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Hirakawa, Yoshihiro","creatorNameLang":"en"},{"creatorName":"ヒラカワ, ヨシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"平川, 義弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000060735976","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060735976"},{"nameIdentifier":"0000-0003-4999-2648","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-4999-2648"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Aoki, Kodai","creatorNameLang":"en"},{"creatorName":"アオキ, コウダイ","creatorNameLang":"ja-Kana"},{"creatorName":"青木, 晃大","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Oh, Jun-Seok","creatorNameLang":"en"},{"creatorName":"オ, ジュンソク","creatorNameLang":"ja-Kana"},{"creatorName":"呉, 準席","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090533779","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090533779"},{"nameIdentifier":"0000-0003-3227-8544","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-3227-8544"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Shirafuji, Tatsuru","creatorNameLang":"en"},{"creatorName":"シラフジ, タツル","creatorNameLang":"ja-Kana"},{"creatorName":"白藤, 立","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010235757","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010235757"},{"nameIdentifier":"0000-0002-5218-5022","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5218-5022"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nakamura, Hiroaki","creatorNameLang":"en"},{"creatorName":"ナカムラ, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"中村, 博亮","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000060227931","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060227931"},{"nameIdentifier":"0000-0002-5380-5000","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5380-5000"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2021 Shimatani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. https://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"プラズマ照射","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"骨再生","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究グループは、骨欠損部位に照射可能なペンシルタイプの「低温大気圧プラズマ照射装置」を共同で開発し、患部へプラズマ照射することにより骨再生が促進することを明らかにしました。本研究成果は、医療分野において骨折治癒期間の短縮や難治性骨折、巨大骨欠損の確実かつ効率的な治療の実現に貢献することが期待できます。近年、プラズマ発生に関する理論・技術の革新に伴い、幅広い分野でプラズマ照射が応用されるようになり、特に生体組織に直接プラズマを照射することにより皮膚疾患の治癒・再生が促進される現象が報告されるなど、革新的医療技術としての期待が高まってきています。本研究グループはこの現象を骨折部の治癒促進に応用することで骨再生の促進や骨癒合期間の短縮が可能ではないかと考えました。今回、本研究グループは、ウサギで尺骨欠損モデルを用い、骨欠損部へ低温大気圧プラズマ照射をした群としない群で新生骨の再生に違いが出るかどうか比較しました。その結果、プラズマ照射時間を5分、10分、15分と変えたプラズマ照射群では、どれも新生骨の再生が確認されました。また、10分照射した群で、コントロールのプラズマを含まないガスを照射した群に比べて8週間後に最も新生骨量が多くなる結果が得られました。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, NTAPP can provide a new treatment opportunity in regenerative medicine. Here, we investigated the impact of NTAPP on bone regeneration using a large bone defect in New Zealand White rabbits and a simple atmospheric pressure plasma (helium microplasma jet). We observed the recovery progress of the large bone defects by X-ray imaging over eight weeks after surgery. The X-ray results showed a clear difference in the occupancy of the new bone of the large bone defect among groups with different plasma treatment times, whereas the new bone occupancy was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the plasma untreated control group. Using H&E and Masson trichrome stains, nucleated cells were uniformly observed, and no inclusion was confirmed, respectively, in the groups of plasma treatment. We concluded the critical large bone defect were filled with new bone. Overall, these results suggest that NTAPP is promising for fracture treatment.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"The authors are highly grateful to JSPS KAKENHI (Grant Number JP19K03811).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1371/journal.pone.0255861"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"e0255861","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-27"}],"displaytype":"detail","filename":"19326203-16-10-e0255861.pdf","filesize":[{"value":"7.8 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020446/files/19326203-16-10-e0255861.pdf"},"version_id":"c1c6fac0-bd22-4218-953f-fa330ed79ca4"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Research Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-10-27"},"publish_date":"2021-10-27","publish_status":"0","recid":"2020446","relation_version_is_last":true,"title":["In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T02:36:13.219824+00:00"}