{"created":"2024-10-31T02:35:35.921637+00:00","id":2020440,"links":{},"metadata":{"_buckets":{"deposit":"3c166c6c-42ba-428c-a16f-a1751a94542e"},"_deposit":{"created_by":7,"id":"2020440","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020440"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020440","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Absorption Characteristics of Vertebrate Non-Visual Opsin, Opn3","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Sugihara, Tomohiro","creatorNameLang":"en"},{"creatorName":"スギハラ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"杉原, 智博","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nagata, Takashi","creatorNameLang":"en"},{"creatorName":"ナガタ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"永田, 崇","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090589962","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090589962/"},{"nameIdentifier":"0000-0001-5379-2951","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-5379-2951"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Stanford University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Mason, Benjamin","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, Japan Science and Technology Agency(JST), Precursory Research for Embryonic Science and Technology(PRESTO)","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Koyanagi, Mitsumasa","creatorNameLang":"en"},{"creatorName":"コヤナギ, ミツマサ","creatorNameLang":"ja-Kana"},{"creatorName":"小柳, 光正","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030379276","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030379276"},{"nameIdentifier":"mkoyanagi","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/mkoyanagi"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Terakita, Akihisa","creatorNameLang":"en"},{"creatorName":"テラキタ, アキヒサ","creatorNameLang":"ja-Kana"},{"creatorName":"寺北, 明久","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030212062","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030212062"},{"nameIdentifier":"0000-0002-8379-8913","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-8379-8913"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2016 Sugihara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"ロドプシン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"分光感度","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"非視覚光受容","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"オプシンの多様性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"眼外光受容","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"rhodopsin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"specctral sensitivity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nonvisual function","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"opsin diversity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"extraocular photoreception","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Most animals possess multiple opsins which sense light for visual and non-visual functions. Here, we show spectral characteristics of non-visual opsins, vertebrate Opn3s, which are widely distributed among vertebrates. We successfully expressed zebrafish Opn3 in mammalian cultured cells and measured its absorption spectrum spectroscopically. When incubated with 11-cis retinal, zebrafish Opn3 formed a blue-sensitive photopigment with an absorption maximum around 465 nm. The Opn3 converts to an all-trans retinal-bearing photoproduct with an absorption spectrum similar to the dark state following brief blue-light irradiation. The photoproduct experienced a remarkable blue-shift, with changes in position of the isosbestic point, during further irradiation. We then used a cAMP-dependent luciferase reporter assay to investigate light-dependent cAMP responses in cultured cells expressing zebrafish, pufferfish, anole and chicken Opn3. The wild type opsins did not produce responses, but cells expressing chimera mutants (WT Opn3s in which the third intracellular loops were replaced with the third intracellular loop of a Gs-coupled jellyfish opsin) displayed light-dependent changes in cAMP. The results suggest that Opn3 is capable of activating G protein(s) in a light-dependent manner. Finally, we used this assay to measure the relative wavelength-dependent response of cells expressing Opn3 chimeras to multiple quantally-matched stimuli. The inferred spectral sensitivity curve of zebrafish Opn3 accurately matched the measured absorption spectrum. We were unable to estimate the spectral sensitivity curve of mouse or anole Opn3, but, like zebrafish Opn3, the chicken and pufferfish Opn3-JiL3 chimeras also formed blue-sensitive pigments. These findings suggest that vertebrate Opn3s may form blue-sensitive G protein-coupled pigments. Further, we suggest that the method described here, combining a cAMP-dependent luciferase reporter assay with chimeric opsins possessing the third intracellular loop of jellyfish opsin, is a versatile approach for estimating absorption spectra of opsins with unknown signaling cascades or for which absorption spectra are difficult to obtain.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This work was supported by Grants-in-aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (nos 23247011, 15H05777 to AT and nos 20770057, 22687005, 15K14568 to MK), Human Frontier Science Program (to AT), PRESTO from the Japan Science and Technology Agency (JST) (to MK), and the National Science Foundation (no 1158967 to BM)","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1371/journal.pone.0161215"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"e0161215","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-13"}],"displaytype":"detail","filename":"111F0000011-11-8-e0161215.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020440/files/111F0000011-11-8-e0161215.pdf"},"version_id":"155c5353-0e39-48f0-93df-ddbee821828a"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Absorption Characteristics of Vertebrate Non-Visual Opsin, Opn3","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-13"},"publish_date":"2017-09-13","publish_status":"0","recid":"2020440","relation_version_is_last":true,"title":["Absorption Characteristics of Vertebrate Non-Visual Opsin, Opn3"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T02:35:45.253235+00:00"}