{"created":"2024-10-31T02:35:31.220507+00:00","id":2020439,"links":{},"metadata":{"_buckets":{"deposit":"972a13b2-2401-4c9c-88a7-e7e3826ef95a"},"_deposit":{"created_by":7,"id":"2020439","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020439"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020439","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kawano-Yamashita, Emi","creatorNameLang":"en"},{"creatorName":"カワノ-ヤマシタ, エミ","creatorNameLang":"ja-Kana"},{"creatorName":"川野-山下, 絵美","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000080804583","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000080804583/"},{"nameIdentifier":"read0114287","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/read0114287"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, The OCU Advanced Research Institute for Natural Science and Technology(OCARINA), Japan Science and Technology Agency(JST), Precursory Research for Embryonic Science and Technology(PRESTO)","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Koyanagi, Mitsumasa","creatorNameLang":"en"},{"creatorName":"コヤナギ, ミツマサ","creatorNameLang":"ja-Kana"},{"creatorName":"小柳, 光正","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030379276","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030379276"},{"nameIdentifier":"mkoyanagi","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/mkoyanagi"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Wada, Seiji","creatorNameLang":"en"},{"creatorName":"ワダ, セイジ","creatorNameLang":"ja-Kana"},{"creatorName":"和田, 清二","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090747320","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090747320/"},{"nameIdentifier":"o2o5","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/o2o5"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Tsukamoto, Hisao","creatorNameLang":"en"},{"creatorName":"ツカモト, ヒサオ","creatorNameLang":"ja-Kana"},{"creatorName":"塚本, 寿夫","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090579814","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090579814/"},{"nameIdentifier":"tsuka","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/tsuka"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nagata, Takashi","creatorNameLang":"en"},{"creatorName":"ナガタ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"永田, 崇","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090589962","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090589962/"},{"nameIdentifier":"0000-0001-5379-2951","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-5379-2951"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, The OCU Advanced Research Institute for Natural Science and Technology(OCARINA)","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Terakita, Akihisa","creatorNameLang":"en"},{"creatorName":"テラキタ, アキヒサ","creatorNameLang":"ja-Kana"},{"creatorName":"寺北, 明久","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030212062","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030212062"},{"nameIdentifier":"0000-0002-8379-8913","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-8379-8913"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2015 Kawano-Yamashita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"松果体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"光受容","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"オプシン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Gタンパク質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"トラスデューシン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"pineal organ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"photoreecption","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"opsin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"G protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transducin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Pineal organs of lower vertebrates contain several kinds of photosensitive molecules, opsins that are suggested to be involved in different light-regulated physiological functions. We previously reported that parapinopsin is an ultraviolet (UV)-sensitive opsin that underlies hyperpolarization of the pineal photoreceptor cells of lower vertebrates to achieve pineal wavelength discrimination. Although, parapinopsin is phylogenetically close to vertebrate visual opsins, it exhibits a property similar to invertebrate visual opsins and melanopsin: the photoproduct of parapinopsin is stable and reverts to the original dark states, demonstrating the nature of bistable pigments. Therefore, it is of evolutionary interest to identify a phototransduction cascade driven by parapinopsin and to compare it with that in vertebrate visual cells. Here, we showed that parapinopsin is coupled to vertebrate visual G protein transducin in the pufferfish, zebrafish, and lamprey pineal organs. Biochemical analyses demonstrated that parapinopsins activated transducin in vitro in a light-dependent manner, similar to vertebrate visual opsins. Interestingly, transducin activation by parapinopsin was provoked and terminated by UV- and subsequent orange-lights irradiations, respectively, due to the bistable nature of parapinopsin, which could contribute to a wavelength-dependent control of a second messenger level in the cell as a unique optogenetic tool. Immunohistochemical examination revealed that parapinopsin was colocalized with Gt2 in the teleost, which possesses rod and cone types of transducin, Gt1, and Gt2. On the other hand, in the lamprey, which does not possess the Gt2 gene, in situ hybridization suggested that parapinopsin-expressing photoreceptor cells contained Gt1 type transducin GtS, indicating that lamprey parapinopsin may use GtS in place of Gt2. Because it is widely accepted that vertebrate visual opsins having a bleaching nature have evolved from non-bleaching opsins similar to parapinopsin, these results implied that ancestral bistable opsins might acquire coupling to the transducin-mediated cascade and achieve light-dependent hyperpolarizing response of the photoreceptor cells.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This work was supported by Grants-in-aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (nos 23247011, 15H05777 to A.T., nos 20770057, 22687005 to M.K. and no 14J40094 to E.K.-Y.), Human Frontier Science Program (to A.T.), PRESTO from the Japan Science and Technology Agency (JST) (to M.K.), and Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to E.K.-Y.)","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1371/journal.pone.0141280"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-10-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"e0141280","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"111F0000011-10-10-e0141280.pdf","filesize":[{"value":"4.9 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020439/files/111F0000011-10-10-e0141280.pdf"},"version_id":"3b34aa39-d54b-45dd-88f3-2b1ad44c1747"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-15"},"publish_date":"2017-09-15","publish_status":"0","recid":"2020439","relation_version_is_last":true,"title":["Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T02:35:39.916798+00:00"}