{"created":"2024-10-31T01:48:36.480699+00:00","id":2020121,"links":{},"metadata":{"_buckets":{"deposit":"90ceec97-accc-4066-96b6-f32d6728f62c"},"_deposit":{"created_by":7,"id":"2020121","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020121"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020121","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Nara Women's University, Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Komura, Tomomi","creatorNameLang":"en"},{"creatorName":"コムラ, トモミ","creatorNameLang":"ja-Kana"},{"creatorName":"小村, 智美","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010736515","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010736515"},{"nameIdentifier":"1025","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/1025"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tokai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yamanaka, Mikihiro","creatorNameLang":"en"},{"creatorName":"ヤマナカ, ミキヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"山中, 幹宏","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000070767946","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000070767946"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Shimane University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nishimura, Kohji","creatorNameLang":"en"},{"creatorName":"ニシムラ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"西村, 浩二","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030304257","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030304257"},{"nameIdentifier":"knishimu","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/knishimu"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Air Water Biodesign Inc.","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Hara, Keita","creatorNameLang":"en"},{"creatorName":"ハラ, ケイタ","creatorNameLang":"ja-Kana"},{"creatorName":"原, 圭太","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tezukayamagakuin University, Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshikazu","creatorNameLang":"en"},{"creatorName":"ニシカワ, ヨシカズ","creatorNameLang":"ja-Kana"},{"creatorName":"西川, 禎一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000060183539","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060183539"},{"nameIdentifier":"0000-0001-9453-0739","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-9453-0739"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2021 The Author(s).","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"線虫","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"最終糖化産物","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"advanced glycation end products","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"AGEs","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究グループは、老化研究のモデル動物としてよく用いられる『線虫』を用いて、生体内で特定の蛍光を発する物質が加齢に伴い増加することを見出しました。その蛍光は最終糖化産物(advanced glycation end products: AGEs)に由来するもので、線虫の余命とも相関していることがわかりました。本モデルの測定技術は、今後、抗糖化素材の探索・評価への活用が期待されます。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"To assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibited longer life expectancy. When proteins extracted from worms were incubated with sugars, the fluorescence intensity and the concentration of advanced glycation end products (AGEs) increased over time. Ribose enhanced these changes not only in vitro but also in vivo. The glycation blocker rifampicin suppressed this rise in fluorescence. High-resolution mass spectrometry revealed that vitellogenins accumulated in old worms, and glycated vitellogenins emitted six-fold higher fluorescence than naive vitellogenins. The increase in fluorescence with ageing originates from glycated substances, and therefore could serve as a useful noninvasive biomarker of AGEs. C. elegans can serve as a new model to look for anti-AGE factors and to study the relationship between AGEs and senescence.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This study was supported in part by the following sources: a Grant-in-Aid for Young Scientists (No. 19K15788) from the Japan Society for the Promotion of Science (T.K.), the Futaba Research Grant Program of the Futaba Foundation (T.K.), the Mishima Kaiun Memorial Foundation (T.K.), and the Nara Women's University Intramural Grant for Project Research in 2017 and 2018 (T.K.).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research ","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1038/s41514-021-00061-y"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2056-3973","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"12","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"npj Aging and Mechanisms of Disease","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-06-11"}],"displaytype":"detail","filename":"20563973-7-1-12.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020121/files/20563973-7-1-12.pdf"},"version_id":"dacbbecc-1cdf-479d-a139-70d34ace47cc"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-06-11"},"publish_date":"2021-06-11","publish_status":"0","recid":"2020121","relation_version_is_last":true,"title":["Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T01:48:47.978080+00:00"}