{"created":"2024-10-31T01:46:38.959820+00:00","id":2020097,"links":{},"metadata":{"_buckets":{"deposit":"37749a2c-e5b3-4b86-938c-263195eecaec"},"_deposit":{"created_by":7,"id":"2020097","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2020097"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02020097","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kobayashi, Kohei","creatorNameLang":"en"},{"creatorName":"コバヤシ, コウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"小林, 昂平","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Kanazawa University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kodera, Noriyuki","creatorNameLang":"en"},{"creatorName":"コデラ, ノリユキ","creatorNameLang":"ja-Kana"},{"creatorName":"古寺, 哲幸","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030584635","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030584635"},{"nameIdentifier":"0000-0003-4880-8423","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-4880-8423"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, Rikkyo University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kasai, Taishi","creatorNameLang":"en"},{"creatorName":"カサイ, タイシ","creatorNameLang":"ja-Kana"},{"creatorName":"笠井, 大司","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"7000023400","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/7000023400"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Tahara, Yuhei O.","creatorNameLang":"en"},{"creatorName":"タハラ, ユウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"田原, 悠平","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0002-2736-9233","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-2736-9233"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Toyonaga, Takuma","creatorNameLang":"en"},{"creatorName":"トヨナガ, タクマ","creatorNameLang":"ja-Kana"},{"creatorName":"豊永, 拓真","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0002-2632-4375","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-2632-4375"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Mizutani, Masaki","creatorNameLang":"en"},{"creatorName":"ミズタニ, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"水谷, 雅希","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000060886274","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000060886274"},{"nameIdentifier":"0000-0001-7950-4866","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-7950-4866"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, Nagaoka University of Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Fujiwara, Ikuko","creatorNameLang":"en"},{"creatorName":"フジワラ, イクコ","creatorNameLang":"ja-Kana"},{"creatorName":"藤原, 郁子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010742075","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010742075"},{"nameIdentifier":"0000-0002-9361-636X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-9361-636X"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Kanazawa University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Ando, Toshio","creatorNameLang":"en"},{"creatorName":"アンドウ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"安藤, 敏夫","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050184320","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050184320"},{"nameIdentifier":"0000-0001-8819-154X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-8819-154X"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Miyata, Makoto","creatorNameLang":"en"},{"creatorName":"ミヤタ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"宮田, 真人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050209912","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050209912"},{"nameIdentifier":"0000-0002-7478-7390","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-7478-7390"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© author(s).","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"高速原子間力顕微鏡","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"分子モーター","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP合成酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"マイコプラズマ・モービレ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"AFM","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"probing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pathogenic bacteria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATPase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"class Mollicutes","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究チームは、最小の細菌である「マイコプラズマ・モービレ」が滑走するための分子モーターの動きを検出することに世界で初めて成功しました。この発見は、ナノスケールのデバイスや医薬品の開発への応用が期待されます。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Mycoplasma mobile, a parasitic bacterium, glides on solid surfaces, such as animal cells and glass, by a special mechanism. This process is driven by the force generated through ATP hydrolysis on an internal structure. However, the spatial and temporal behaviors of the internal structures in living cells are unclear. In this study, we detected the movements of the internal structure by scanning cells immobilized on a glass substrate using high-speed atomic force microscopy (HS-AFM). By scanning the surface of a cell, we succeeded in visualizing particles, 2 nm in height and aligned mostly along the cell axis with a pitch of 31.5 nm, consistent with previously reported features based on electron microscopy. Movements of individual particles were then analyzed by HS-AFM. In the presence of sodium azide, the average speed of particle movements was reduced, suggesting that movement is linked to ATP hydrolysis. Partial inhibition of the reaction by sodium azide enabled us to analyze particle behavior in detail, showing that the particles move 9 nm right, relative to the gliding direction, and 2 nm into the cell interior in 330 ms and then return to their original position, based on ATP hydrolysis.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This work was supported by Grants-in-Aid for Scientific Research (A) (MEXT KAKENHI, grant number JP17H01544), JST CREST (grant number JPMJCR19S5), Osaka City University (OCU) Strategic Research Grant 2018 for top priority research, and by a Grantin-Aid for the Fugaku Trust for Medicinal Research to M. Miyata.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1128/mBio.00040-21"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2150-7511","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"e00040-21","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"mBio","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-06-04"}],"displaytype":"detail","filename":"21507511-12-3-e00040-21.pdf","filesize":[{"value":"4.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2020097/files/21507511-12-3-e00040-21.pdf"},"version_id":"c35b4d51-17ab-4028-8886-a0b2ba238a8b"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Research Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-06-04"},"publish_date":"2021-06-04","publish_status":"0","recid":"2020097","relation_version_is_last":true,"title":["Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T01:46:48.930090+00:00"}