{"created":"2024-10-31T01:00:44.057288+00:00","id":2019707,"links":{},"metadata":{"_buckets":{"deposit":"1203d836-2a92-400f-b6ea-4550b050047b"},"_deposit":{"created_by":7,"id":"2019707","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2019707"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02019707","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Non-heat-stressed Method to Isolate Hepatic Stellate Cells From Highly Steatotic Tumor-bearing Liver Using CD49a","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka Metropolitan University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Cheng, Yi","creatorNameLang":"en"},{"creatorName":"程, 禕","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0002-3288-6841","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-3288-6841"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka Metropolitan University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yamagishi, Ryota","creatorNameLang":"en"},{"creatorName":"ヤマギシ, リョウタ","creatorNameLang":"ja-Kana"},{"creatorName":"山岸, 良多","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030793145","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030793145"},{"nameIdentifier":"0000-0002-6827-2382","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-6827-2382"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nonaka, Yoshiki","creatorNameLang":"en"},{"creatorName":"ノナカ, ヨシキ","creatorNameLang":"ja-Kana"},{"creatorName":"野中, 允幾","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka Metropolitan University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Sato-Matsubara, Misako","creatorNameLang":"en"},{"creatorName":"マツバラ(サトウ), ミサコ","creatorNameLang":"ja-Kana"},{"creatorName":"松原(佐藤), 三佐子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000000635120","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000635120"},{"nameIdentifier":"0000-0003-0593-7305","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-0593-7305"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka Metropolitan University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kawada, Norifumi","creatorNameLang":"en"},{"creatorName":"カワダ, ノリフミ","creatorNameLang":"ja-Kana"},{"creatorName":"河田, 則文","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000030271191","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030271191"},{"nameIdentifier":"Hepatology","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/Hepatology"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka Metropolitan University, Japan Agency for Medical Research and Development","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Ohtani, Naoko","creatorNameLang":"en"},{"creatorName":"オオタニ, ナオコ","creatorNameLang":"ja-Kana"},{"creatorName":"大谷, 直子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050275195","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050275195"},{"nameIdentifier":"0000-0001-8934-0797","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-8934-0797"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 - 非営利 - 改変禁止 4.0 国際 ","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-nd/4.0/ "},{"subitem_rights":"Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International ","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-nd/4.0/ "},{"subitem_rights":"© 2022 The Authors.","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"肝がん","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"高度脂肪肝","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"肝星細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究グループは、肝がんを伴う高度脂肪肝から肝星細胞を単離する方法を開発しました。本研究成果により、これまで解析されていなかったがん微小環境における肝星細胞の1細胞レベルの性質変化が明らかになり、がん予防や治療につながることが期待されます。がんの組織はがん細胞そのものだけでなく、肝星細胞など様々な種類の細胞種が集まってがん微小環境を構成しています。本研究グループが以前明らかにしたように、肝がんでは腫瘍部に存在する肝星細胞から分泌される因子が抗腫瘍免疫を抑制し、肝がんを進展させます。そのため、肝がんの特性を理解するには、様々な状態の肝臓から肝星細胞を効率良く単離して、微小環境における肝星細胞の性質を解析する必要があります。近年、シングルセルRNAシーケンス解析の手法が発達し、1細胞レベルでの細胞の性質解析が可能となりました。これまでマウスの正常肝から肝星細胞を単離する方法は報告されていましたが、内臓脂肪を多く蓄積した肥満マウスの脂肪肝や、脂肪肝を素地とする腫瘍に存在する肝星細胞を高純度かつ高効率に単離する方法は開発されていませんでした。また、細胞単離時に使用する酵素処理を37度で実施すると、熱ストレス関連遺伝子が有意に発現上昇し、アーティファクトとなることも報告されていました。そこで本研究グループは、内臓脂肪を多く蓄積した脂肪肝の腫瘍部から、熱ストレス関連アーティファクトを極力抑えた6度という低温で酵素処理し、かつ、密度勾配遠心分離法と肝星細胞の細胞表面分子CD49aを用いたセルソーティングを組み合わせた方法により、高純度に効率よく肝星細胞を単離する方法の開発に成功しました。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The number of patients with non-viral, nonalcoholic steatohepatitis-associated liver cancer has been increasing with the prevalence of obesity. Increasing evidence shows that the tumor microenvironment (TME) is crucial for tumor progression. ...","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This study was funded by AMED (Japan Agency for Medical Research and Development), under grant numbers JP20gm1010009 (Naoko Ohtani and Norifumi Kawada), JP20fk0210050 (Norifumi Kawada) and JP19ck0106260 (Naoko Ohtani). This study was also supported by the Japan Society for the Promotion of Science (JSPS) under grant numbers 19H04002 (Naoko Ohtani), 19H03641 (Norifumi Kawada), 21K07968 (Misako Sato-Matsubara), and 20K16309 (Ryota Yamagishi) as well as a grant from Takeda Science Foundation (Naoko Ohtani) and Yakult Bio-Science Foundation (Naoko Ohtani), and Research Grant of the Princess Takamatsu Cancer Research Fund 18-25003 (Naoko Ohtani).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1016/j.jcmgh.2022.07.006"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2352-345X","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"966.e9","bibliographicPageStart":"964","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Cellular and Molecular Gastroenterology and Hepatology","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-07"}],"displaytype":"detail","filename":"2352345x-14-4-964.pdf","filesize":[{"value":"4.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2019707/files/2352345x-14-4-964.pdf"},"version_id":"b1ff8b80-5b8a-42d9-9bd7-72cde02f9022"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Research Letter","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Non-heat-stressed Method to Isolate Hepatic Stellate Cells From Highly Steatotic Tumor-bearing Liver Using CD49a","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-10-07"},"publish_date":"2022-10-07","publish_status":"0","recid":"2019707","relation_version_is_last":true,"title":["Non-heat-stressed Method to Isolate Hepatic Stellate Cells From Highly Steatotic Tumor-bearing Liver Using CD49a"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T01:00:54.677341+00:00"}