{"created":"2024-10-31T00:53:37.424861+00:00","id":2019661,"links":{},"metadata":{"_buckets":{"deposit":"e9d3713f-5da4-4b74-b467-62a837e995c5"},"_deposit":{"created_by":7,"id":"2019661","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2019661"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02019661","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Seki, Soichiro","creatorNameLang":"en"},{"creatorName":"セキ, ソウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"関, 荘一郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0003-4706-4696","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-4706-4696"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nakaniwa, Tetsuko","creatorNameLang":"en"},{"creatorName":"ナカニワ, テツコ","creatorNameLang":"ja-Kana"},{"creatorName":"仲庭, 哲津子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000080569070","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000080569070"},{"nameIdentifier":"0000-0003-3637-9860","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-3637-9860"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Thermo Fisher Scientific","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Castro-Hartmann, Pablo","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Thermo Fisher Scientific","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Sader, Kasim","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kawamoto, Akihiro","creatorNameLang":"en"},{"creatorName":"カワモト, アキヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"川本, 晃大","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090631523","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090631523"},{"nameIdentifier":"0000-0002-7380-0127","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-7380-0127"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Tanaka, Hideaki","creatorNameLang":"en"},{"creatorName":"タナカ, ヒデアキ","creatorNameLang":"ja-Kana"},{"creatorName":"田中, 秀明","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000040346169","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000040346169"},{"nameIdentifier":"read0092284","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/read0092284"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Thermo Fisher Scientific","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Qian, Pu","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kurisu, Genji","creatorNameLang":"en"},{"creatorName":"クリス, ゲンジ","creatorNameLang":"ja-Kana"},{"creatorName":"栗栖, 源嗣","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000090294131","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000090294131"},{"nameIdentifier":"0000-0002-5354-0807","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5354-0807"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University, Osaka Metropolitan University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Fujii, Ritsuko","creatorNameLang":"en"},{"creatorName":"フジイ, リツコ","creatorNameLang":"ja-Kana"},{"creatorName":"藤井, 律子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000080351740","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000080351740"},{"nameIdentifier":"0000-0003-1492-109X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-1492-109X"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2022 The Author(s). Published by Elsevier B.V.","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"Codium fragile","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryo-EM","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Light-harvesting complex","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Siphonaxanthin–chlorophyll a","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"b-binding protein (SCP)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intermonomer chlorophyll b macrocluster","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究グループは、高分解能の電子顕微鏡を用いた解析により、海洋性緑藻ミルの光合成アンテナと呼ばれるタンパク質内での色素の構造と結合環境を初めて明らかにしました。その結果、海水中で得られる唯一の光である青緑色光を効率よく光合成に利用する分子メカニズムがより鮮明になりました。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Light-harvesting complex II (LHCII) present in plants and green algae absorbs solar energy to promote photochemical reactions. A marine green macroalga, Codium fragile, exhibits the unique characteristic of absorbing blue-green light from the sun during photochemical reactions while being underwater owing to the presence of pigment-altered LHCII called siphonaxanthin–chlorophyll a/b-binding protein (SCP). In this study, we determined the structure of SCP at a resolution of 2.78 Å using cryogenic electron microscopy. SCP has a trimeric structure, wherein each monomer containing two lutein and two chlorophyll a molecules in the plant-type LHCII are replaced by siphonaxanthin and its ester and two chlorophyll b molecules, respectively. Siphonaxanthin occupies the binding site in SCP having a polarity in the trimeric inner core, and exhibits a distorted conjugated chain comprising a carbonyl group hydrogen bonded to a cysteine residue of apoprotein. These features suggest that the siphonaxanthin molecule is responsible for the characteristic green absorption of SCP. The replaced chlorophyll b molecules extend the region of the stromal side chlorophyll b cluster, spanning two adjacent monomers.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"The grant-in-aid of the Sasakura Enviro-Science Foundation (to SS), the OCU Strategic Research Grant for Basic Researches (to RF), and the JST-CREST under grant number JPMJCR20E1 (GK) were appreciated. SS thanks the Sunbor : SUNTORY Foundation for Life Sciences Bioorganic Research Institute for a scholarship (FY2020-2023). This work was performed in part under the Collaborative Research Program of the Institute for Protein Research, Osaka University (CR-21-02 to RF), and the joint research program of the Artificial Photosynthesis, Osaka City University (FY2018 to TN).","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1016/j.bbadva.2022.100064"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2667-1603","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"100064","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"BBA Advances","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-12"}],"displaytype":"detail","filename":"26671603-2-100064.pdf","filesize":[{"value":"9.0 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2019661/files/26671603-2-100064.pdf"},"version_id":"da242eb9-2203-4a51-bac3-1563d9e76115"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Research article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-12-12"},"publish_date":"2022-12-12","publish_status":"0","recid":"2019661","relation_version_is_last":true,"title":["Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T00:53:46.652162+00:00"}