{"created":"2024-10-31T00:50:00.696890+00:00","id":2019615,"links":{},"metadata":{"_buckets":{"deposit":"cd333fcb-1b8d-4651-aa4b-021ac4163861"},"_deposit":{"created_by":7,"id":"2019615","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2019615"},"status":"published"},"_oai":{"id":"oai:ocu-omu.repo.nii.ac.jp:02019615","sets":["1623632832836:1721355153493"]},"author_link":[],"item_1617186331708":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Ueda, Yuki","creatorNameLang":"en"},{"creatorName":"ウエダ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"上田, 裕貴","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Tahara, Yuhei O.","creatorNameLang":"en"},{"creatorName":"タハラ, ユウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"田原, 悠平","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"0000-0002-2736-9233","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-2736-9233"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Miyata, Makoto","creatorNameLang":"en"},{"creatorName":"ミヤタ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"宮田, 真人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000050209912","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050209912"},{"nameIdentifier":"0000-0002-7478-7390","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-7478-7390"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Ogita, Akira","creatorNameLang":"en"},{"creatorName":"オギタ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"荻田, 亮","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000000244624","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000244624"},{"nameIdentifier":"0000-0002-1694-2356","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-1694-2356"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Yamaguchi, Yoshihiro","creatorNameLang":"en"},{"creatorName":"ヤマグチ, ヨシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"山口, 良弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000000737009","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000737009"},{"nameIdentifier":"0000-0003-4663-6963","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-4663-6963"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Tanaka, Toshio","creatorNameLang":"en"},{"creatorName":"タナカ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"田中, 俊雄","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010137185","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010137185"},{"nameIdentifier":"read0020847","nameIdentifierScheme":"RESEARCHMAP","nameIdentifierURI":"https://researchmap.jp/read0020847"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka City University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Fujita, Ken-ichi","creatorNameLang":"en"},{"creatorName":"フジタ, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"藤田, 憲一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000010285281","nameIdentifierScheme":"KAKEN2","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010285281"},{"nameIdentifier":"0000-0001-8496-7435","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0001-8496-7435"}]}]},"item_1617186499011":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"クリエイティブ・コモンズ　表示 4.0 国際","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"},{"subitem_rights":"© 2021 authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"抗真菌薬","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"薬剤耐性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"薬剤排出ポンプ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"急速凍結レプリカ電子顕微鏡","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"antifungal","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nagilactone E","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"drug resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"multidrug efflux pump","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"quick-freeze deep-etch electron microscopy","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Nagilactone E, an antifungal agent derived from the root bark of Podocarpus nagi, inhibits 1,3-β glucan synthesis; however, its inhibitory activity is weak. Anethole, the principal component of anise oil, enhances the antifungal activity of nagilactone E. We aimed to determine the combinatorial effect and underlying mechanisms of action of nagilactone E and anethole against the budding yeast Saccharomyces cerevisiae. Analyses using gene-deficient strains showed that the multidrug efflux pump PDR5 is associated with nagilactone E resistance; its transcription was gradually restricted in cells treated with the drug combination for a prolonged duration but not in nagilactone-E-treated cells. Green-fluorescent-protein-tagged Pdr5p was intensively expressed and localized on the plasma membrane of nagilactone-E-treated cells but not in drug-combination-treated cells. Quick-freeze deep-etch electron microscopy revealed the smoothening of intertwined fiber structures on the cell surface of drug-combination-treated cells and spheroplasts, indicating a decline in cell wall components and loss of cell wall strength. Anethole enhanced the antifungal activity of nagilactone E by enabling its retention within cells, thereby accelerating cell wall damage. The combination of nagilactone E and anethole can be employed in clinical settings as an antifungal, as well as a food preservative to restrict food spoilage.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This research was partly funded by the Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (C) 16K08299 and 21K07029.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_1617186783814":{"attribute_name":"識別子","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.3390/antibiotics10050537"}]},"item_1617186920753":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2079-6382","subitem_source_identifier_type":"ISSN"}]},"item_1617187056579":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"537","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Antibiotics","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1722232185104":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-28"}],"displaytype":"detail","filename":"20796382-10-5-537.pdf","filesize":[{"value":"34.9 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://ocu-omu.repo.nii.ac.jp/record/2019615/files/20796382-10-5-537.pdf"},"version_id":"e95a116d-041d-4c4d-b87d-4ee23aea942b"}]},"item_1722577087681":{"attribute_name":"記事種別等","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1725590125188":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_title":"Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae","item_type_id":"40002","owner":"7","path":["1721355153493"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-09-28"},"publish_date":"2021-09-28","publish_status":"0","recid":"2019615","relation_version_is_last":true,"title":["Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-31T00:50:09.716391+00:00"}